Coding

Part:BBa_K5301000:Design

Designed by: Xing Zhang   Group: iGEM24_BNU-China   (2024-09-21)


MSP1E3D1 is a genetically engineered protein, which mimics the function of ApoA-I.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 115
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 115
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 115
    Illegal BglII site found at 741
    Illegal XhoI site found at 637
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 115
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 115
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Control the length of MSP and reference the ApoA-1 sequence to enable efficient binding with phospholipids.


Source

It's genetically engineered based on Apolipoprotein A-1 (ApoA-1).

References